A 76-acre old growth wooded parcel located on the southern border of Hocking County close to Ash Cave State Park. This area is the reported location of one of the last native Ohio bison. The bison is now a memory but the wild beauty of Bison Hollow remains. These protected woodlands forever protect the singing waters of the east branch of Queer Creek, act as a buffer to Ash Cave and the nearby Buckeye and North Country Trails, and provide a home for locally rare plants such as the recently discovered Autumn Coral Root Orchid.

An 80-acre parcel located adjacent to Bison Hollow, features many of the same conservation values. Carpenter Hollow is located in northern Vinton County on the Hocking County line.

Located directly across the road from Ash Cave State Park this 19-acre site preserves an adjacent wooded parcel. The House site provides a buffer to the park site and helps maintain the wild character of the area.

Cackley Swamp is located adjacent to Cooper Hollow Wildlife Area in southern Jackson County near the town of Pyro. This property includes 220 acres with frontage on CH&D Road, Pyro Road and Harmon Road. The preserve includes primary, secondary and tertiary streams which are part of the Symmes Creek watershed surrounded by emergent and deep water wetland. Much of this area is too shallow for a canoe, but too deep for hiking boots. Ducks, muskrat and beavers love the wetlands. Otters are reported to be residents as well. This land was purchased by AOA through a grant from the US Fish and Wildlife Service.

This 32-acre parcel near Sugar Grove in Fairfield County consists of a small valley ringed by spectacular outcrops of Black Hand Sandstone. Donated by the heirs of Bertram and Josephine Kleinmaier, this treasured natural area is a component of the nearby Rhododendron Hollow State Nature Preserve. Both of these wooded sites harbor healthy populations of the state threatened Great Rhododendron and associated plants such as Partridge Berry, Wintergreen, Pink Moccasin Flower, Indian Pipe and Walking Fern. In a wet spring seep the locally rare Swamp Saxifrage grows in association with a dense forest and a concentration of Skunk Cabbage.

The Sickles Preserve is a 70-acre former goat farm which is entirely surrounded by Zaleski State Forest. Its acquisition prevented development of this key in-holding. The property contains hardwood forests and an old-field meadow. Hewett Fork (tributary to Raccoon Creek) winds through the center of the property. There are several acres of button-bush wetland along the creek. The Moonville Rail Trail, a non-motorized trail extending from Zaleski, Ohio to New Marshfield crosses the site. The preserve is located at the end of King Hollow in Waterloo Township, Athens County.

At 1,205 acres Trimble Township Community Forest (TTCF) is AOA’s largest preserve. It provides an important wildlife corridor between the Trimble Wildlife Area and the Wayne National Forest. TTCF was acquired through grants from the Clean Ohio Fund and the Ohio Division of Wildlife. It is managed by AOA in partnership with the Division of Wildlife and the Sunday Creek Watershed Group. TTCF serves as a land lab for Trimble High School, located about a mile away. The property fronts Derthick, Taylor Ridge and Bethel Ridge Roads in Athens County just north of the Town of Glouster. The preserve contains hardwood forest, clean-running streams, and a beaver created wetland complex containing several dams. An expansive network of pre-existing oil-well roads allows easy access for hikers. Public access for hiking and hunting is permitted without charge.